Academic Thesis

Basic information

Name Kutara Kenji
Belonging department
Occupation name
researchmap researcher code B000294859
researchmap agency Okayama University of Science

Title

Ozone water has antibacterial properties in dogs without skin barrier impairment

Bibliography Type

Joint Author

Author

Matsuda A, Ano T, Nakamura Y, Itoi T, Arai K, Kutara K, Sugimoto K, Maeta N.

Summary

Background: Recently, the use of disinfectants to treat bacterial skin diseases in dogs has garnered attention from the perspective of preventing the spread of antibiotic-resistant bacteria. Ozone water is a disinfectant that combines high antibacterial efficacy and safety; however, there have been no reports verifying its effects on skin barrier function in dogs.

Objectives: This study aimed to verify the antibacterial properties and effects of ozone water on skin barrier function in dogs.
Animals: Four beagles for the antibacterial test and six beagles for the barrier function test.
Materials and methods: Purified water, 3 mg/L ozone water and 0.005% and 0.05% sodium hypochlorite were used as test products. Skin bacteria were collected 5 min after a single application of each and cultured to measure the bacterial counts. Trans-epidermal water loss (TEWL) and skin surface hydration (SSH) were measured before and after application once a day for 2 weeks. Additionally, the proliferation of canine epidermal keratinocytes (CPEK) was examined after a 5-min incubation with the test products.
Results: Ozone water and 0.05% sodium hypochlorite significantly reduced the number of skin bacteria. None of the test products worsened TEWL and SSH values. Sodium hypochlorite completely inhibited the proliferation of CPEK, while ozone water did not.
Conclusions and clinical relevance: Ozone water has an antibacterial effect on canine skin comparable to that of 0.05% sodium hypochlorite, but does not inhibit keratinocyte proliferation in vitro. Thus, ozone water has the potential to be used as a safe and effective disinfectant for canine skin.

Magazine(name)

Veterinary Dermatology

Publisher

Volume

36

Number Of Pages

3

StartingPage

283

EndingPage

290

Date of Issue

2025/06

Referee

Exist

Invited

Not exist

Language

English

Thesis Type

Research papers (academic journals)

ISSN

DOI

doi: 10.1111/vde.13339.

NAID

PMID

URL

J-GLOBAL ID

arXiv ID

ORCID Put Code

DBLP ID