Based on theoretical consideration, the effect of enzyme and substrate concentration on the hydrolysis of crystalline cellulose by cellulase was investigated experimentally. The result suggested that synergistic effect for the hydrolysis of crystalline cellulose, MCC, was closely related to kinetic constants of each cellulase component. From the hydrolysis of MCC by mixed solutions of MCCase and CMCase, which were obtained by partial purification of crude Meicelase, synergistic effect was found to depend on especially Vmax / Km value. In this study, when the amount ratio of MCCase to CMCase was 7 to 1, Vmax / Km value was about 29.4 (μg total sugar / mg protein / min %w/v) and synergistic effect became the highest.
