論文

基本情報

氏名 河野 真二
氏名(カナ) カワノ シンジ
氏名(英語) Kawano Shinji
所属 生命科学部 生物科学科
職名 准教授
researchmap研究者コード B000335013
researchmap機関 岡山理科大学

題名

The impact of the DNA topoisomerase IIβ C-terminal region on the selective degradation induced by ICRF-193 treatment

単著・共著の別

共著

著者

Shinji Kawano and Shogo Ikeda

概要

ICRF-193, a catalytic inhibitor of DNA topoisomerase II (TOP2), induces the formation of the TOP2 closed-clamp intermediate. Only the ICRF-193-induced topoisomerase IIb (TOP2B) closed clamp is known to be selectively and rapidly degraded in vertebrates, but the details are unknown. In this study, we focused on the C-terminal domain (CTD) of TOP2B, which regulates its nuclear dynamics, and sought the region that affects the ICRF-193-induced TOP2B closed-clamp degradation. Using a CTD-swapping mutant between topoisomerase IIa(TOP2A) and TOP2B, we found that the CTD of TOP2B, but not that of TOP2A, is involved in the TOP2B closed-clamp degradation. Furthermore, we identified the C-terminal region (CTR) of TOP2B (amino acids 1,570-1,621) as a domain that affects TOP2B closed clamp degradation using CTR truncation mutant (DCTR). A transcription inhibitor inhibited the ICRF-193-induced TOP2B closed-clamp degradation, but the TOP2B DCTR closed-clamp degradation was not. In addition, the results of co-immunoprecipitation and immunofluorescence staining showed that the proximity of TOP2B and RNA polymerase II on chromatin in the presence of ICRF-193 tended to be reduced by the lack of TOP2B CTR. Taken together, our data indicate that the TOP2B CTR is involved in the transcription-dependent TOP2B closed-clamp degradation induced by ICRF-193.

発表雑誌等の名称

Genes to Cells

出版者

開始ページ

終了ページ

発行又は発表の年月

2025/03

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

ID:DOI

https://doi.org/10.1111/gtc.70017

ID:NAID(CiNiiのID)

ID:PMID

URL

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID