論文

基本情報

氏名 河野 真二
氏名(カナ) カワノ シンジ
氏名(英語) Kawano Shinji
所属 生命科学部 生物科学科
職名 准教授
researchmap研究者コード B000335013
researchmap機関 岡山理科大学

題名

Ascorbic acid and thiol antioxidants suppress spontaneous mutagenesis in a Cu,Zn-superoxide dismutase-deficient mutant of Saccharomyces cerevisiae.

単著・共著の別

著者

K. Nagira, S. Tamura, S. Kawano, S. Ikeda

概要

Cu,Zn-superoxide dismutase (SOD1) is a critical enzyme in the cellular antioxidant system. The yeast Saccharomyces cerevisiae SOD1 mutant (SOD1Δ) exhibits a moderate mutator phenotype under aerobic conditions. The mutation frequency of a SOD1Δ strain determined by a CAN1 forward-mutation assay was about 12-fold higher than that of the parental strain. Base substitutions G·CT·A, G·CA·T, and A·TC·G were most commonly observed in CAN1 mutants, indicating that the mutations are caused mainly by oxidative DNA damage. The mutation frequency of SOD1Δ was reduced in a dose-dependent manner by cultivating it in the presence of ascorbic acid, implying that the SOD1Δ mutant can be used as a tester strain for small molecule antioxidants. Exogenous glutathione and N-acetylcystein also alleviated the mutator phenotype. The results indicate that ascorbic acid and thiol antioxidants are able to efficiently protect cells against oxidative damage-induced mutagenesis. In this assay, no apparent mutation suppression was seen for other categories of antioxidants including resveratrol, Trolox and melatonin.

発表雑誌等の名称

Genes Environ.

出版者

35

開始ページ

110

終了ページ

114

発行又は発表の年月

2013/11

査読の有無

有り

招待の有無

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

ID:DOI

ID:NAID(CiNiiのID)

ID:PMID

URL

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID