論文

基本情報

氏名 河野 真二
氏名(カナ) カワノ シンジ
氏名(英語) Kawano Shinji
所属 生命科学部 生物科学科
職名 准教授
researchmap研究者コード B000335013
researchmap機関 岡山理科大学

題名

Suppressive Effects of Natural Compounds on Methionine Auxotrophy of a Cu,Zn-Superoxide Dismutase-Deficient Mutant of Saccharomyces cerevisiae

単著・共著の別

著者

概要

Yeast lacking Cu,Zn-superoxide dismutase (sod1Δ) exhibits methionine (Met) auxotrophy, primarily due to the depletion of NADPH required in the sulfur assimilation pathway by oxidative stress. In this study, we measured the ability of natural compounds, including ingredients in foods and dietary supplements, to restore the cell growth of sod1Δ in liquid and solid media without Met. Homocysteine at around 0.1 mM fully suppressed auxotrophy but in excess (> 0.4 mM) had a harmful effect. Methionine sulfoxide at concentrations from 0.05 to 0.8 mM and S-adenosylmethione (0.05 to 6.2 mM) completely relieved the growth defects caused by sod1Δ. Ascorbic acid (1 to 50 mM) restored sod1Δ growth, indicating that the action of this antioxidant could improve Met biosynthesis. However, ascorbic acid derivatives, ascorbic acid 2-glucoside and dehydroascorbic acid, did not show any activity. The suppressive effects of cysteine, N-acetylcysteine, and glutathione peaked at 0.1 mM, 0.5 mM, and 0.05 mM, respectively, but an excess of these agents was less effective. Suppression by dithiothreitol confirms that the thiol group is responsible for the amelioration of Met biosynthesis. No growth was restored for other categories of antioxidants including polyphenols, Trolox, melatonin, astaxanthin, and others.

発表雑誌等の名称

Food Science and Technology Research

出版者

開始ページ

終了ページ

発行又は発表の年月

2015/01

査読の有無

有り

招待の有無

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

ID:DOI

ID:NAID(CiNiiのID)

ID:PMID

URL

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID