論文

基本情報

氏名 河野 真二
氏名(カナ) カワノ シンジ
氏名(英語) Kawano Shinji
所属 生命科学部 生物科学科
職名 准教授
researchmap研究者コード B000335013
researchmap機関 岡山理科大学

題名

DNA binding activity of rat DNA topoisomerase IIα C-terminal domain contributes to efficient DNA catenation in vitro

単著・共著の別

著者

Shinji Kawano, Yuri Kato, Natsumi Okada, Kuniaki Sano, Ken Tsutsui, Kimiko M. Tsutsui and Shogo Ikeda

概要

DNA topoisomerase IIα (topo IIα) is an essential enzyme for resolution of DNA topologies arising in DNA metabolic reactions. In proliferating cells, topo II activities of DNA catenation or decatenation are required for condensation of chromosomes and segregation of chromatids. Recent studies suggest that the C-terminal domain (CTD) of human topo IIα is required for localization to mitotic chromosomes. Here, we show that the CTD of topo IIα is also associated with efficient DNA catenation in vitro, based on comparison of wild-type (WT) rat topo IIα and its deletion mutants. Unlike WT, the CTD truncated mutant (ΔCTD) lacked linear DNA binding activity, but could bind to negatively supercoiled DNA similarly to WT. The CTD alone showed linear DNA-binding activity. ΔCTD mediated formation of a DNA catenane in the presence of polyethylene glycol, which enhances macromolecular association. These results indicate that DNA-binding activity in the CTD of topo IIα concentrates the enzyme in the vicinity of condensed DNA and allows topo IIα to efficiently form a DNA catenane.

発表雑誌等の名称

The Journal of Biochemistry

出版者

159

3

開始ページ

363

終了ページ

369

発行又は発表の年月

2016/03

査読の有無

有り

招待の有無

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

ID:DOI

ID:NAID(CiNiiのID)

ID:PMID

URL

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID