論文

基本情報

氏名 久楽 賢治
氏名(カナ) クタラ ケンジ
氏名(英語) Kutara Kenji
所属 獣医学部 獣医学科
職名 講師
researchmap研究者コード B000294859
researchmap機関 岡山理科大学

題名

Daily topical application of chlorhexidine gluconate to the skin in dogs and its impact on skin barriers and cytotoxicity

単著・共著の別

共著

著者

Matsuda A, Nakamura Y, Itoi T, Arai K, Kutara K, Sugimoto K, Maeta N.

概要

In recent years, the importance of using local disinfectants instead of systemic antibiotics for the treatment of infectious skin diseases to prevent the emergence of resistant bacteria has become widely recognized. Chlorhexidine gluconate (CHG) is commonly used in veterinary antibacterial shampoos; however, the daily topical application of diluted CHG solutions has also been adopted. Despite its widespread use, few studies have investigated the effects of CHG on the canine skin barrier. This study examined the skin barrier-damaging effects of CHG in dogs by applying diluted CHG (0.05%, 0.5%, and 4%) daily to six healthy dogs for 2 weeks. At the end of the trial, transepidermal water loss (TEWL), skin surface hydration (SSH), and number of skin bacteria were evaluated. Additionally, the cytotoxicity of CHG to the canine progenitor epidermal keratinocytes (CPEK) was examined using the water-soluble tetrazolium salt (WST-8) assay. Continuous application of 0.5% and 4% CHG to the skin led to a significant reduction in the number of skin bacteria. Additionally, at 4% CHG, a notable increase in TEWL and a decrease in SSH was observed. The WST assay revealed cytotoxicity of CHG at concentrations of 0.05%. In conclusion, although daily topical application of 4% CHG demonstrated the potential to disrupt the skin barrier, 0.5% CHG demonstrated sufficient antimicrobial activity without skin barrier disruption. Additionally, when treating dogs' skin with a compromised stratum corneum, use of lower concentration of CHG is suitable.

発表雑誌等の名称

Journal of Veterinary Medicine Science

出版者

87

3

開始ページ

257

終了ページ

262

発行又は発表の年月

2025/05

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

ID:DOI

doi: 10.1292/jvms.24-0311.

ID:NAID(CiNiiのID)

ID:PMID

URL

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID