論文

基本情報

氏名 齋藤 文代
氏名(カナ) サイトウ フミヨ
氏名(英語) Saito Fumiyo
所属 獣医学部 獣医学科
職名 准教授
researchmap研究者コード B000357497
researchmap機関 岡山理科大学

単著・共著の別

共著

著者

Kiyotaka Arai;Fumiyo Saito;Masashi Miyazaki;Haruto Kushige;Yayoi Izu;Noritaka Maeta;Kazuaki Yamazoe

概要

Several methods have been developed to generate neurons from other cell types for performing regeneration therapy and in vitro studies of central nerve disease. Small molecules (SMs) can efficiently induce neuronal features in human and rodent fibroblasts without transgenes. Although canines have been used as a spontaneous disease model of human central nerve, efficient neuronal reprogramming method of canine cells have not been well established. We aimed to induce neuronal features in adult canine dermal fibroblasts (ACDFs) by SMs and assess the permanency of these changes. ACDFs treated with eight SMs developed a round-shaped cell body with branching processes and expressed neuronal proteins, including βIII-tubulin, microtubule-associated protein 2 (MAP2), and neurofilament-medium. Transcriptome profiling revealed the upregulation of neuron-related genes, such as SNAP25 and GRIA4, and downregulation of fibroblast-related genes, such as COL12A1 and CCN5. Calcium fluorescent imaging demonstrated an increase in intracellular Ca2+ concentration upon stimulation with glutamate and KCl. Although neuronal features were induced similarly in basement membrane extract droplet culture, they diminished after culturing without SMs or in vivo transplantation into an injured spinal cord. In conclusion, SMs temporarily induce neuronal features in ACDFs. However, the analysis of bottlenecks in the neuronal induction is crucial for optimizing the process.

発表雑誌等の名称

International Journal of Molecular Sciences

出版者

開始ページ

終了ページ

発行又は発表の年月

2023/10

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

ID:DOI

https://doi.org/10.3390/ijms242115804

ID:NAID(CiNiiのID)

ID:PMID

URL

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID