論文

基本情報

氏名 齋藤 文代
氏名(カナ) サイトウ フミヨ
氏名(英語) Saito Fumiyo
所属 獣医学部 獣医学科
職名 准教授
researchmap研究者コード B000357497
researchmap機関 岡山理科大学

単著・共著の別

共著

著者

Hiroko Ikeda;Eikichi Ihara;Kosuke Takeya;Koji Mukai;Manabu Onimaru;Kenoki Ouchida;Yoshitaka Hata;Xiaopeng Bai;Yoshimasa Tanaka;Taisuke Sasaki;Fumiyo Saito;Masumi Eto;Jiro Nakayama;Yoshinao Oda;Masafumi Nakamura;Haruhiro Inoue;Yoshihiro Ogawa

概要

Background

Achalasia is an esophageal motility disorder with an unknown etiology. We aimed to determine the pathogenesis of achalasia by studying alterations in esophageal smooth muscle contraction and the associated inflammatory response, and evaluate the role of esophageal microbiota in achalasia development.

Methods

We analyzed esophageal mucosa and lower esophageal sphincter (LES) samples, obtained from patients with type II achalasia who underwent peroral endoscopic myotomy. Esophageal conditioned media obtained from patients were transferred into the mouse esophagus to determine whether the esophageal intraluminal environment is associated with achalasia.

Results

Approximately 30% of 20-kDa myosin light chains (LC20) was phosphorylated in LES from the control group under resting and stimulated conditions, whereas less than 10% of LC20 phosphorylation was detected in achalasia under all conditions. The hypophosphorylation of LC20 in achalasia was associated with the downregulation of the myosin phosphatase-inhibitor protein CPI-17. Th17-related cytokines, including IL-17A, IL-17F, IL-22, and IL-23A, were significantly upregulated in achalasia. α-Diversity index of esophageal microbiota and the proportion of several microbes, including Actinomyces and Dialister, increased in achalasia. Actinomyces levels positively correlated with IL-23A levels, whereas Dialister levels were positively associated with IL-17A, IL-17F, and IL-22 levels. Esophageal IL-17F levels increased in mice after oral administration of the conditioned media.

発表雑誌等の名称

Journal of Gastroenterology

出版者

開始ページ

終了ページ

発行又は発表の年月

2024/03

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

ID:DOI

https://doi.org/10.1007/s00535-024-02088-w

ID:NAID(CiNiiのID)

ID:PMID

URL

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID